The Glycohemoglobin analyzer is a laboratory instrument that dilutes the whole blood specimen with Hemolysis & Wash Solution, and then injects a small volume of this specimen onto a Variant Column. Separation is achieved by utilizing differences in ionic interactions between the cation exchange group on the column resin surface and the hemoglobin components. The hemoglobin fractions (designated as A1a, A1b, F, LA1c+, SA1c, A0, and H-V0, H-V1, H-V2) are subsequently removed from the column by performing a step-wise elution using the varied salt concentrations in the Elution Buffers HSi Variant 1, 2, and 3.
The separated hemoglobin components pass through the LED photometer flow cell where the analyzer measures changes in absorbance at 415 nm. The analyzer integrates and reduces the raw data, and then calculates the relative percentages of each hemoglobin fraction. The Total Area of the SA1c is divided by the sum of the total areas of all peaks up to and including the A0 to obtain a raw SA1c percentage. This uncorrected result is substituted as the “x” value in the linear regression formula determined during calibration. The analyzer prints the final numerical results and plots a chromatogram showing changes in absorbance versus retention time for each peak fraction.
Tosoh G7 HPLC
Tosoh G7 HPLC
Bayer DCA 2000 Plus